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Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA▿

机译：质粒DNA的体外甲基化提高了顽固性蜡状芽孢杆菌和魏氏杆菌的转化效率

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摘要

Digestion patterns of chromosomal DNAs of Bacillus cereus and Bacillus weihenstephanensis strains suggest that Sau3AI-type restriction modification systems are widely present among the isolates tested. In vitro methylation of plasmid DNA was used to enhance poor plasmid transfer upon electroporation to recalcitrant strains that carry Sau3AI restriction barriers.

机译：蜡样芽孢杆菌和维恩芽孢杆菌菌株的染色体DNA的消化模式表明，在测试的分离物中广泛存在Sau3AI型限制性修饰系统。质粒DNA的体外甲基化用于增强电穿孔后对携带Sau3AI限制性屏障的顽固菌株的不良质粒转移。

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Groot, Masja Nierop; Nieboer, Frank; Abee, Tjakko;
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年度 2008
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1. Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA [J] . Masja Nierop Groot, Frank Nieboer, Tjakko Abee Applied and Environmental Microbiology . 2008,第24期

机译：质粒DNA的体外甲基化提高了顽固的蜡状芽孢杆菌和魏氏杆菌分离株的转化效率
2. Complete Sequence Analysis of Novel Plasmids from Emetic and Periodontal Bacillus cereus Isolates Reveals a Common Evolutionary History among the B. cereus-Group Plasmids, Including Bacillus anthracis pXO1 [J] . David A. Rasko, M. J. Rosovitz, Ole Andreas ?kstad, Journal of bacteriology . 2007,第1期

机译：从催吐和牙周芽孢杆菌分离株的新型质粒的完整序列分析揭示了蜡状芽孢杆菌组质粒，包括炭疽芽孢杆菌pXO1的共同进化史。
3. Diversity of pulsed-field gel electrophoresis patterns of cereulide-producing isolates of Bacillus cereus and Bacillus weihenstephanensis [J] . Virginie Castiaux, Elise NGuessan, Izabela Swiecicka, FEMS Microbiology Letters . 2014,第2期

机译：蜡状芽孢杆菌和魏杆菌的蜡状芽孢杆菌分离株的脉冲场凝胶电泳图谱多样性
4. Optimization of a protoplast transformation method for Bacillus subtilis, Bacillus megaterium, and Bacillus cereus by a plasmid pHIS1525.SplipA [C] . A. Mirabdollah, S. Alinezhad, E. Feuk-Lagerstedt, International Conference on Environmental, Industrial and Applied Microbiology . 2009

机译：通过质粒pHIS1525252525-优化枯草芽孢杆菌，芽孢杆菌和芽孢杆菌和芽孢杆菌的原生质体变换方法的优化
5. The Dissection of Β-Lactam Resistance in Bacillus Anthracis, Bacillus Cereus, and Bacillus Thuringiensis [D] . Nauta, Kelsie Marie. 2021

机译：芽孢杆菌，芽孢杆菌，芽孢杆菌和芽孢杆菌的β-内酰胺抗性解剖
6. Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA [O] . Masja Nierop Groot, Frank Nieboer, Tjakko Abee 2008

机译：质粒DNA的体外甲基化提高顽固性蜡状芽孢杆菌和魏氏杆菌分离株的转化效率
7. Bacillus anthracis and Bacillus cereus PcrA Helicases Can Support DNA Unwinding and In Vitro Rolling-Circle Replication of Plasmid pT181 of Staphylococcus aureus [O] . Anand, Syam P., Mitra, Poulami, Naqvi, Asma, 2004

机译：炭疽芽孢杆菌和蜡状芽孢杆菌PcrA解旋酶可支持DNA展开和金黄色葡萄球菌质粒pT181的体外转染循环复制
8. Genome Sequence of Bacillus cereus ATCC 10987 Reveals Metabolic Adaptations and a Large Plasmid Related to Bacillus anthracis pXO1 [R] . Rasko, D. A. , Ravel, J. , Okstad, O. A. , 2004

机译：蜡状芽孢杆菌aTCC 10987的基因组序列揭示了代谢适应和与炭疽杆菌pXO1相关的大质粒

Enhanced Transformation Efficiency of Recalcitrant Bacillus cereus and Bacillus weihenstephanensis Isolates upon In Vitro Methylation of Plasmid DNA▿

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